Butterfield's Phosphate Buffered Dilution Water (BFD) 25ml Bottle
Butterfield's Phosphate Buffered Dilution Water is used for preparation of microbiological dilution blanks
One case contains 100 units.
For Stock Solution
Monopotassium phosphate 34g
Distilled Water 500mL
Adjust pH to 7.2 with 1N NaOH. Bring volume up to 1L with distilled water. Sterilize 15 min at 121C. Store in refrigerator
Take 1.25mL of above stock solution and add to 1L of distilled water. Dispense into 9mL or 90mL tubes. Sterilize 15 min at 121C
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