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Demi-Fraser Broth (DFB)

Tryptone 5g

Peptone 5g

Beef Extract 5g

Yeast Extract 5g

Sodium Chloride 20g

Disodium Phosphate 9.6g

Monopotassium Phosphate 1.35g

Esculin 1g

Lithium Chloride 3g

Nalidixic Acid 0.01g

Acriflavin 0.012g

Ferric Ammonium Citrate 0.01g

Final pH: 7.2 ± 0.2 at 25°C

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