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Triple Sugar Iron Agar (TSI)


The Triple Sugar Iron (TSI) test is a microbiological test named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. It is often used to differentiate enteric bacteria including Salmonella and Shigella


Enzymatic Digest of Casein 5g

Enzymatic Digest of Animal Tissue 5g

Sodium Thiosulfate 0.3g

Sucrose 10g

Sodium Chloride 5g

Ferric Ammonium Citrate 0.2g

Phenol Red 0.025

Agar 13.5g

Final pH: 7.3 ± 0.2 at 25°C


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