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SIMUL-qPCR E. coli O157:H7 with GFP Assay


Food processors and associated laboratories can use the SIMUL-qPCR System as a quick and reliable method for detecting the Top 7 Shiga toxin-producing E. coli in raw beef trim, raw ground beef, leafy greens, dried hemp flower and spunbonded polyolefin sampling sheets. All SIMUL-qPCR System assays are designed to have the same instrument run time allowing simultaneous identification of all SIMUL-qPCR System assays. In addition, each assay utilizes the power of multiplexing several targets during the same run. The SIMUL-qPCR Top 7 STEC Assay incorporates a multiplex approach to identifying E. coli O157:H7, O26, O45, O103, O111, O121, O145, Shiga toxin and intimin genes. The SIMUL-qPCR E. coli O157:H7 with GFP Assay incorporates a real-time PCR approach to identifying E. coli O157:H7 and brings an added layer of assurance with the addition of the Green Fluorescent Protein (GFP) positive control.

Fluorescence-based reporter systems are generally utilized for microbial analysis due to their easily measured and distinguished phenotypes. One of the most commonly used fluorescent reporters is the green fluorescent protein (GFP) as it is well-characterized and contains many convenient inherent properties, such as spontaneous fluorescence. Recently, GFP reporters have been added to quality control strains, which differentiates a control strain from actual food contaminants or naturally occurring microorganisms.

This test kit is for the detection of Escherichia coli (O157:H7), and the reported labeled strain is UVQC E. coli (O157:H7), which contains a Green Fluorescent Protein (GFP) marker.

One kit contains 96 tests. One case contains 6 kits.

One kit contains 96 tests. One case contains 6 kits. 


    • Concurrent detection of both the target bacteria and a reporter labeled QC (catalog number: SMRT-UVQCECH7-020) of the target bacteria: By detecting both the target bacteria and the QC strain, the assay can confirm the presence of the target bacteria while also detecting any potential issues with the assay, such as sample or reagent contamination. This ensures the results are accurate, reducing the risk of false positives.
    • Fast and accurate detection of harmful pathogens: Our assay is designed to detect Shiga toxin-producing E.coli quickly and accurately (as little as 12 hours), enabling the food industry to take immediate action in the event of a positive test result.
    • Easy to use: The assay is easy to use and can be performed by personnel with minimal training.
    • Cost-effective: The assay is cost-effective, as it eliminates the need for additional testing to confirm the results, reducing the overall cost of testing. This assay incorporates a single enrichment media can be used to test for E. coli O157:H7 and Salmonella.


    • Number of Tests: 96 test/kit
    • Targets: E. coli O157:H7, Green Fluorescent Protein (GFP)
    • Enrichment time: 10-18 hours
    • Limit of Detection: 10,000 CFU/mL after Enrichment
    • PCR Run Time: 55 minutes
    • Validations: AOAC PTM 022001
    • Validated Matrices: Raw Beef Trim, Raw Ground Beef, Spunbonded Polyolefin Sampling Sheets, Leafy Greens
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